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XLD

XLD (Xylose Lysine Deoxycholate) Agar is a selective and differential culture medium widely used in clinical and industrial microbiology laboratories for the isolation and identification of Gram-negative enteric pathogens, particularly Salmonella and Shigella species. The medium relies on the fermentation of xylose, lysine decarboxylation, and hydrogen sulfide production to differentiate target organisms from non-pathogenic enteric bacteria. XLD Agar is formulated to suppress the growth of Gram-positive organisms and coliforms while allowing the growth of Salmonella and Shigella, which produce characteristic colony morphologies: red colonies with black centers (for H2S-positive Salmonella) or red colonies without black centers (for Shigella). The medium is available in two primary forms: dehydrated powder for laboratory preparation and ready-to-use pre-poured plates. Dehydrated powder, such as the 10 L and 500 mL sizes supplied by 3G Electric, offers a shelf life of 2 years when stored properly, making it suitable for laboratories that prepare media in bulk. Pre-poured plates, such as the 100 x 15 mm size, are provided as solid media in sterile packaging with a 90-day shelf life, ideal for immediate use in high-throughput testing environments. XLD Agar is manufactured under controlled conditions to ensure batch-to-batch consistency, with each lot tested for performance characteristics including growth promotion, inhibition, and colony morphology. The medium complies with standard formulations such as those described in the U.S. Pharmacopeia (USP) and other pharmacopeial methods for microbial limit testing. 3G Electric supplies XLD Agar products from established manufacturers, offering multiple SKUs to accommodate different laboratory workflows and volume requirements. The product line includes powder in bottle containers (SKU 39M301 for 10 L and SKU 39M303 for 500 mL) and pre-poured plates (SKU 45H464 for 100 x 15 mm). Each SKU is identified by a manufacturer part number (e.g., 7166-10kg, 7166-500g, MP7142) for traceability and ordering accuracy. For procurement specialists and laboratory managers, XLD Agar is a standard medium for food safety testing, water quality analysis, and clinical diagnostics where enteric pathogen detection is required. The medium's selectivity is achieved through the inclusion of deoxycholate and sodium thiosulfate, which inhibit Gram-positive bacteria and provide a substrate for H2S production. The differential properties rely on the pH indicator phenol red, which turns red in alkaline conditions (lysine decarboxylation) and yellow in acidic conditions (xylose fermentation). Colonies of Salmonella typically appear red with black centers due to H2S production, while Shigella colonies remain red without blackening. Non-pathogenic coliforms may produce yellow colonies. XLD Agar is a critical component of microbiological quality control programs in industries such as food processing, pharmaceuticals, and environmental monitoring. 3G Electric supports global procurement by offering these products with logistics coordination, ensuring that laboratories receive media with documented shelf life and proper handling instructions. The availability of multiple pack sizes allows users to select the format that aligns with their testing frequency and storage capacity.

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Tentang XLD

XLD (Xylose Lysine Deoxycholate) Agar is a selective and differential culture medium widely used in clinical and industrial microbiology laboratories for the isolation and identification of Gram-negative enteric pathogens, particularly Salmonella and Shigella species. The medium relies on the fermentation of xylose, lysine decarboxylation, and hydrogen sulfide production to differentiate target organisms from non-pathogenic enteric bacteria. XLD Agar is formulated to suppress the growth of Gram-positive organisms and coliforms while allowing the growth of Salmonella and Shigella, which produce characteristic colony morphologies: red colonies with black centers (for H2S-positive Salmonella) or red colonies without black centers (for Shigella). The medium is available in two primary forms: dehydrated powder for laboratory preparation and ready-to-use pre-poured plates. Dehydrated powder, such as the 10 L and 500 mL sizes supplied by 3G Electric, offers a shelf life of 2 years when stored properly, making it suitable for laboratories that prepare media in bulk. Pre-poured plates, such as the 100 x 15 mm size, are provided as solid media in sterile packaging with a 90-day shelf life, ideal for immediate use in high-throughput testing environments. XLD Agar is manufactured under controlled conditions to ensure batch-to-batch consistency, with each lot tested for performance characteristics including growth promotion, inhibition, and colony morphology. The medium complies with standard formulations such as those described in the U.S. Pharmacopeia (USP) and other pharmacopeial methods for microbial limit testing. 3G Electric supplies XLD Agar products from established manufacturers, offering multiple SKUs to accommodate different laboratory workflows and volume requirements. The product line includes powder in bottle containers (SKU 39M301 for 10 L and SKU 39M303 for 500 mL) and pre-poured plates (SKU 45H464 for 100 x 15 mm). Each SKU is identified by a manufacturer part number (e.g., 7166-10kg, 7166-500g, MP7142) for traceability and ordering accuracy. For procurement specialists and laboratory managers, XLD Agar is a standard medium for food safety testing, water quality analysis, and clinical diagnostics where enteric pathogen detection is required. The medium's selectivity is achieved through the inclusion of deoxycholate and sodium thiosulfate, which inhibit Gram-positive bacteria and provide a substrate for H2S production. The differential properties rely on the pH indicator phenol red, which turns red in alkaline conditions (lysine decarboxylation) and yellow in acidic conditions (xylose fermentation). Colonies of Salmonella typically appear red with black centers due to H2S production, while Shigella colonies remain red without blackening. Non-pathogenic coliforms may produce yellow colonies. XLD Agar is a critical component of microbiological quality control programs in industries such as food processing, pharmaceuticals, and environmental monitoring. 3G Electric supports global procurement by offering these products with logistics coordination, ensuring that laboratories receive media with documented shelf life and proper handling instructions. The availability of multiple pack sizes allows users to select the format that aligns with their testing frequency and storage capacity.

Aplikasi Umum

  • Food safety testing — XLD Agar (SKU 39M301) for isolation of Salmonella from processed meat and dairy samples.
  • Clinical diagnostics — XLD Agar pre-poured plates (SKU 45H464) for stool specimen screening in hospital microbiology labs.
  • Water quality analysis — XLD Agar powder (SKU 39M303) for detecting enteric pathogens in recreational and drinking water.
  • Pharmaceutical quality control — XLD Agar (SKU 39M301) for microbial limit testing of raw materials and finished products.
  • Environmental monitoring — XLD Agar plates (SKU 45H464) for surface and air sampling in cleanroom facilities.
  • Veterinary microbiology — XLD Agar (SKU 39M303) for Salmonella screening in animal feed and fecal samples.

Pertanyaan yang Sering Diajukan

Pertanyaan umum tentang XLD dari 3G Electric.

What is the typical shelf life of XLD Agar powder supplied by 3G Electric?

XLD Agar in dehydrated powder form, such as SKU 39M301 (10 L) and SKU 39M303 (500 mL), has a shelf life of 2 years when stored in a cool, dry environment away from direct sunlight and moisture. The powder should be kept in its original sealed bottle to prevent caking or contamination. After opening, the remaining powder should be used within a reasonable timeframe to maintain performance, though the manufacturer's stated shelf life applies to unopened containers. Pre-poured plates (SKU 45H464) have a shorter shelf life of 90 days due to the hydrated state and require refrigeration at 2-8°C.

How does XLD Agar differentiate Salmonella from Shigella?

XLD Agar differentiates Salmonella from Shigella based on biochemical reactions. Both organisms are Gram-negative and grow on the medium, but Salmonella typically ferments xylose initially, producing acid, then decarboxylates lysine, reverting the pH to alkaline. This results in red colonies. Additionally, Salmonella produces hydrogen sulfide (H2S) from sodium thiosulfate, forming black centers in the colonies. Shigella does not produce H2S and may not decarboxylate lysine, so colonies appear red without black centers. Non-pathogenic coliforms that ferment xylose without lysine decarboxylation produce yellow colonies.

What are the storage conditions for XLD Agar pre-poured plates from 3G Electric?

XLD Agar pre-poured plates (SKU 45H464, 100 x 15 mm) should be stored at 2-8°C in a refrigerator to maintain their solid consistency and prevent dehydration or contamination. The plates are packaged in sterile sleeves and should be kept in their original packaging until use. Avoid freezing, as ice crystal formation can damage the agar structure. The shelf life is 90 days from the date of manufacture, and plates should be inspected for cracks, discoloration, or visible contamination before use. Allow plates to reach room temperature before inoculation to avoid condensation.

Can XLD Agar powder be used for preparing media in large volumes?

Yes, XLD Agar powder is designed for laboratory preparation of media in various volumes. SKU 39M301 (10 L) is suitable for high-throughput labs that require bulk media preparation, while SKU 39M303 (500 mL) is ideal for smaller batches or occasional use. The powder must be rehydrated with distilled or deionized water according to the manufacturer's instructions, typically 55-60 g per liter, then heated to boiling to dissolve completely. After sterilization by autoclaving at 121°C for 15 minutes, the medium is cooled to 45-50°C and poured into sterile Petri dishes. The prepared plates should be used within 7 days if stored properly.

What quality control tests are performed on XLD Agar supplied by 3G Electric?

Each lot of XLD Agar is tested for performance characteristics including growth promotion of target organisms (e.g., Salmonella enterica, Shigella flexneri), inhibition of non-target organisms (e.g., Escherichia coli, Enterococcus faecalis), and typical colony morphology. Tests are conducted according to standard methods such as USP <61> or ISO 11133. The medium is also checked for pH (typically 7.4 ± 0.2 at 25°C), sterility, and physical appearance. Certificates of analysis are available upon request for each SKU, providing batch-specific data on these parameters.

Does 3G Electric supply XLD products?

Yes — 3G Electric (S) Pte. Ltd. supplies XLD products. We stock the XLD catalog and supply genuine factory-original products with full manufacturer warranty and technical support.